For making of solid media agar plates, 1.5% agar is added to the media and autoclaved. After autoclaving allow the media to cool up-to 50°C.Transfer the warm media into a petri dishes until it is 1/3 full, loosely close the lid and allow the media to solidify.

Figure 8.1:Equipments and media required for sterilization and growth of bacterial expression system.(A)autoclave (B)autoclaved LB broth (C) E.coli grown in LB broth.