STEP 6: Glyceraldehyde-3-phosphate to 1,3 bis-phospho-glycerate- In this step, one molecule of NADH is produced after oxidation of aldehyde group of glyceraldehyde-3-phosphate with the help of enzyme glyceraldehyde-3-phosphate dehydrogenase .

STEP 7: In this step, phosphate group from 1,3 bis-phosphoglycerate is removed by phosphoglycerate kinase with an acyl phosphate group transfer to ADP to generate ATP molecule.

STEP 8: Conversion of 3-phosphoglycerate to 2-phosphoglycerate- In a two step mechanism, phosphoglycerate mutase catalyzes a reversible shift of phosphoryl group to form 2-phosphoglycerate.

STEP 9: Dehydration of 2-phosphoglycerate to phosphoenol pyruvate- The enzyme enolase catalyzes the dehydration reaction to produce phosphoenol pyruvate , a compound with high phosphoryl group transfer potential.

STEP 10: In the last step of glycolysis, phosphate group from phosphoenol pyruvate is transferred by pyruvate kinase with an acyl phosphate group transfer to ADP to generate ATP molecule.