There is an inverse linear relationship between the logarithm of the electrophoretic mobility and gel concentration .
Example 2: Compare gel percentage vs. expected migration rate (Mr)
Gel % |
1/Gel % |
inv log(1/Gel %)
(i.e. relative Mr ) |
| 2.0 |
0.5 |
3.2 |
| 1.5 |
0.66 |
4.6 |
1.0 |
1.0 |
10.0 |
0.5 |
2.0 |
100.0 |
Assignment
1. What is the function of the agarose gel in electrophoresis ?
a. Stabilizes DNA.
b. Helps in DNA migration.
c. Provides matrix for separation.
d. Provides charge to DNA.
2. What are the functions of the buffer in electrophoresis ?
a. Stabilizes DNA.
b. Provides matrix for separation.
c. Helps in DNA migration
d. Provides ionic charge and pH.
3. What are the functions of the EtBr in agarose gel electrophoresis ?
a. Increases mobility of DNA.
b. Helps in visualizing of DNA.
c. Increases conductivity.
d. Helps in separation of DNA by size.
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