4. Toward which pole does DNA migrate when electric current is run through the gel and why?
a. From +ve pole to -ve pole as DNA is +ve charged.
b. From -ve pole to +ve pole as DNA is +ve charged.
c. From +ve pole to -ve pole as DNA is -ve charged.
d. From -ve pole to +ve pole as DNA is -ve charged.
5. What are the three main factors effecting the migration rate of DNA on grarose gel?
a. DNA concentration, voltage, and buffer concentration.
b. Gel percentage, buffer concentration and voltage.
c. Size of DNA, gel concentration and conformations of DNA.
d. Length of the gel, buffer concentration and DNA concentration.
6. Give the relation between migration rate (Mr) and gel concentration (gel %).
a. log (gel%) α 1/log[ Mr ]
b. log (Mr) α 1/[gel%]
c. 1/log (Mr) α 1/[gel%]
d. log (Mr) α [gel%]
7. Give the relation between migration rate (Mr) and molecular weight (Mw).
a. 1/Mrα 1/ log (Mw)
b. log Mrα 1/ log (Mw)
c. 1/log M r α log (Mw)
d. Mrα 1/ log (Mw)
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