3. Nomenclature of restriction enzyme

Each enzyme is named after the bacterium from which it was isolated using a naming system based on bacterial genus, species and strain. Some examples are given below:

•  Eco R1: E.coli Restriction Endonuclease I

•  StuI: Streptomyces tubercidicus I

•  PstI: Providencias tuartii I

 

4. Applications

Restriction enzymes are used to manipulate DNA for following scientific applications:

•  RFLP– Restriction Fragment Length Polymorphism

•  DNA finger printing

•  Molecular diagnostics

•  Cloning

•  cDNA library construction

•  Population studies

•  Sequencing

•  Gene amplification

•  Finding polymorphism (one clone, one template)