EXPERIMENT

AIM: Clonal propagation by axillary shoot proliferation from nodal explants of Spilanthes acmella

 

EQUIPMENTS: Autoclave, pH-meter, Magnetic stirrers, Magnetic beads, Weighing balance, Laminar-air-flow

 

MATERIALS REQUIRED : Salts and vitamins of Murashige and Skoog's (MS; 1962) , sucrose, agar, conical flasks, measuring cylinders and beakers of various sizes. Reagent glass bottles for storage, spatula, tissue rolls, distilled water. Cotton plugs, aluminium foils, muslin cloth, scissor, media stocks, 1N NaOH, 1N HCl, myo-inositol. Autoclavable polybags, rubber bands, borosil glass test-tubes (150mm x 25mm without rim). Black markers, micropippete, micropipette-tips, test-tube stands, autoclavable baskets, plastic trays, fresh stem cuttings of S. acmella .

Plant Growth regulators (Sigma)

Benzyl amino purine (BAP), 6-furfuryl amino purine (Kinetin), 6-γ γ –Dimethylallylamino purine (2-iP), Naphthalene acetic acid (NAA), Indole-3-butyric acid.

 

PROTOCOL

Figure 6.1: Schematic view of establishment of nodal segment cultures of S. acmella for clonal                      propagation