4. Axillary shoot proliferation vs adventitious shoot proliferation
i. | The axillary shoot proliferation is the most popular approach to clonal propagation of crop plants because the cells of the shoot apex are uniformly diploid and are least susceptible to genotypic changes under culture conditions. |
ii. | Chimeras, whose breakdown is common during adventitious bud proliferation, are perpetuated in shoot-bud culture and, thus, the cause for change in ploidy sometimes. While axillary shoot proliferation favours genetically uniform plant formation. |
iii. | Moreover adventitious bud formation and callusing methods require denovo differentiation of shoot-buds which is not always possible. |
iv. | Further, the axillary shoot proliferation is comparatively a quicker method of shoot multiplication as pre-existing meristem only proliferate into shoots, thus, reducing the time required to form de novo meristem formation. |
5. Axillary shoot proliferation vs conventional method of propagation
i. | The conventional method of vegetative propagation by stem cuttings utilizes the ability of axillary buds to take over the function of main shoot in the absence of a terminal bud. However, the number of cuttings that can be taken from a selected plant in a year is extremely limited because in nature the vegetative growth is periodic. In in vitro conditions, axillary shoot proliferate irrespective of seasons and regions. |
ii. | A minimal size of cuttings required in conventional methods is around 24-30 cm in order to establish a plant from it. Thus, it may restrict the multiplication of plants if the stock of parent plant is limited or if the species is endangered. |
iii. | With axillary shoot proliferation, minimum cutting size required is <1cm, thus, it favours large scale multiplication even with the limited sample. |
iv. | With the axillary shoot proliferation method, juvenile nodal cuttings are made available throughout the year that helps to maintain faster rate of multiplication compare to conventional methods of vegetative propagation where juvenile phase is short lived and with mature cuttings it is difficult to establish propagation as the buds in the axil under go dormancy. |