Study Questions

 

1.  What are the different methods of protoplast fusion? Explain the mechanism involved in PEG and High Ca⁺⁺ method of fusion.

2.  Can you differentiate fusion partners during protoplast fusion?

3.  How would you calculate the frequency of heterokaryon formation (Fusion frequency)?

4.  Differentiate between the following:

• i.  Hybridization and cybridization

ii.  Homokaryons and heterokaryon

iii.  Symmetric fusion and asymmetric fusion

iv.  Spontaneous and induced fusion