Study Questions

 

1.  Define protoplasts. Why the protoplast can be used as a starting material for genetic manipulation.

2.  What is the role of cell wall degrading enzymes?

3.  Name the best source used for protoplast isolation.

4.  Which chemical is considered to be a most important media component for protoplast isolation and culture?

5.  How do you count protoplasts and why it is essential?

6.  List out different protoplast culture techniques. Why do you think a sequential reduction in osmoticum is necessary during culture?