4-3.2.4. Cloning of cDNAs

cDNAs are usually cloned in phage insertion vectors.Bacteriophage vectors offer the following advantageous over plasmid vectors,

•  are more suitable when a large number of recombinants are required for cloning low-abundant mRNAs as recombinant phages are produced by in vitro packaging.

•  can easily store and handle large numbers of phage clones, as compared to the bacterial colonies carrying plasmids.

Plasmid vectors are used extensively for cDNA cloning, particularly in the isolation of the desired cDNA sequence involving the screening of a relatively small number of clones.

Figure 4-3.2.4: Cloning of cDNAs