II. Fill in the blanks with appropriate words and terminology.
1. A ----------- is a nuclease enzyme that cleaves DNA sequence at a random or specific recognition sites known as restriction sites.
2. Restriction endonucleases are named according to the organism in which they were ----- using a system of -------and ----------.
3. Cleavage by an endonuclease creates DNA sequence with either a -------or-------.
4. --------------- is a bifunctional enzyme and functions in mRNA processing and degradation inside the cell.
5. Enzyme that can catalyze the hydrolytic cleavage of phosphodiester bonds in the DNA backbone are known as ---------------.
6. Exonuclease III is a globular enzyme which has ----------------- activity in a double stranded DNA.
7. ----------------- nuclease enzymes can degrade single stranded DNA as well RNA.
8. ---------------- catalyses the cleavage of a phosphate (PO4-2) group from substrate by using a water molecule (hydrolytic cleavage).
9. --------------- shows its optimal activity at pH between 3 and 6.
10. ------------- show their optimal activity at pH of about 10.
11. --------------- catalyzes the transfer of methyl group (-CH3) to its substrate.
12. ------------ catalyses the formation of phosphodiester bond between two deoxynucleotide residues of two DNA strands.
13. E.coli and other bacterial DNA ligase utilizes ------------energy donor.
14. T4 DNA ligase uses ---------- as cofactor.
15. --------------- catalyzes the transfer of a phosphate group (PO4-2 ) from γ position of ATP to the 5' end of either DNA or RNA and nucleoside monophosphate.