I. Choose the correct answer.

1. Type II restriction enzymes require ……………… as cofactor for its activity

(A)  ATP

(B)  Mg²⁺

(C)  Both a and b

(D)  None of the above

2. Restriction enzume BamHI is a

(A)  Phosphatase

(B)  Exonuclease

(C) Endonuclease

(D)  Both b and c

3. DNase enzyme cleaves the following bond:

(A) hydrogen bond

(B)  glycosidic bond

(C)  peptide bond

(D) phosphodiester bond

4 . Exonuclease III has the following activity:

(A)  5' → 3' exonuclease activity

(B)  3'→5' exonuclease activity

(C)  5' → 3' endouclease activity

(C)  3'→5' endouclease activity

5. The optimal activity of acid phosphatase lies between pH range of

(A)  0-1 pH

(B)  3-7 pH

(C)  7-10 pH

(D)  3-6 pH

6. The optimal temperature for RibonucleaseA enzyme activity is-

(A)  30°C

(B)  60°C

(C)  37° C

(D)  42°C

7. Polynucleotide kinase (PNK) activity is inhibited by-

(A)  calcium ion

(B)  potassium ion

(C)  magnesium ion

(D)  ammonium ion