STEP 9: Dehydration of 2-phosphoglycerate to phosphoenol pyruvate- The enzyme enolase catalyzes the dehydration reaction to produce phosphoenol pyruvate, a compound with high phosphoryl group transfer potential.  

STEP 10: In the last step of glycolysis, phosphate group from phosphoenol pyruvate is transferred by pyruvate kinase with an acyl phosphate group transfer to ADP to generate ATP molecule.

 

Regulation of Glycolysis-

1. Uptake of glucose from blood-The level of glucose present in a cell determines the availability of sugar for oxidation via glycolysis. Glucose transport in cell is regulated by several cell surface receptor which are under the control of insulin (Figure 36.5). Insulin upregulates the level of glucose transporters Glut-3 or Glut-4 and increase the uptake of glucose from blood stream. In addition, insulin also regulates breakdown of glycogen to increase the amount of available glucose.