If DNA is double stranded, how it can denature to access the information nucleotide sequence.  DNA double helix can be break open, if it is exposed to the high temperature or titrate with acid or an alkali. During this process, the hydrogen bonding between two strand breaks. This process is known as melting or denaturation. When the denatured DNA is incubated at low temperature, the separated strands reassociate to form duplex DNA. This process is known as renaturation. The denatutation/renaturation kinetics is used to understand the complexicity of the DNA and it has wide application in amplifying the strand using polymerase chain reaction (PCR).