Evidence against the theory:  There are evidences against the mutation theory proposed by Huge De Vries. These objections are as follows:

(i)  The mutation are of rare occurrence. Hence it is difficult to assume that if all animal or plant species could appear solely by mutation.

(ii) The relationship between flower and the insect (such as length of proboscis in insect and position of nectariesd in flower) can not be explained by mutation theory.

(iii) Mutations are recessive where as it is generally the dominant mutations that brings about evolution.