ii. Vacuum Transfer Methods: Compare to the capillary transfer method, vacuum transfer method is more efficient. Complete transfer of DNA from gel to the nylon membrane takes place in couple of hours compared to 16-20hrs in capillary transfer. Setup of the vacuum blot to transfer the DNA from gel to the membrane is given in the Figure 19.3.

1. Treat the gel as described in the step 1-5 of the capillary methods.

2. Cut the nylon membrane and the silicon mask with an opening smaller than the size of the gel.

3. Place the wet 3mm whatman filter paper on the metal grid.

4. Place the nylon membrane on the 3mm whatman filter paper and subsequently put mask.