3. Prepration of RNA sample:

1. Take the RNA sample and make up-to 6.5µl with appropriate quantity.

2. To each sample, add 2.5µl 10x MOPS running buffer, 4.5µl 37% formaldehyde, 11.5µl formamide.

3. Mix by vortexing and briefly spin to collect the sample at the bottom.

4. Inside the hood, add 5µl RNA loading buffer, mix by vortexing and briefly spin to collect the sample at the bottom.

4. Loading of the RNA sample: Fill the agarose denatured gel prepared with the 1x MOPS running buffer. Load the RNA sample into the lane (Figure 18.3).

 

 

Figure 18.3: Steps in Loading of the RNA samples into the denatured agarose gel.