CDPro software suite

CDPro is a suite of programs developed by Sreerama and Woody for analyzing the far-UV CD data [2]. The suite contains three programs, SELCON3, CONTINLL, and CDSSTR and is freely available at http://lamar.colostate.edu/~sreeram/CDPro . The information about the algorithms these programs use can be found elsewhere [1,2,4].

 

Materials:

1. CDPro software suite: CDPro can be downloaded as CDPro.zip file from http://lamar.colostate.edu/~sreeram/CDPro .
2. The far UV CD spectrum to be analyzed.
   
   

Procedure:

1. Extract the files from CDPro.zip in a new directory, CDPro.
   
   
2. Most CD instruments will generate data in ellipticity (millidegrees ).
   
   
3. Correct the protein/peptide spectrum by subtracting the blank spectrum.
   
   
4. Save the corrected spectrum as a text (ASCII) file.
   
   
5. Covert the ellipticity (mdeg) into mean residue ellipticity, [θ] as discussed in previous lecture.
   
   
6. Convert the mean residue ellipticity data into per residue ∆ε units as follows: