Next generation sequencing methods: With the advancement in cloning and requirement for cheap sequencing methodology, several next generation sequencing methods are developed. These have lower cost of sequencing as well increased the output by processing multiple sample at the same time. The next generation DNA sequencing technologies developed as follows-

• Massively parallel signature sequencing (MPSS)

• Polony sequencing

• 454 pyrosequencing

• Illumina (Solexa) sequencing

• SOLiD sequencing

• Ion semiconductor sequencing

• DNA nanoball sequencing

• Heliscope single molecule sequencing

• Single molecule real time (SMRT) sequencing