3. Movement is too fast-In few cases, the movement of a compound is too fast and does not give time to interact with the matrix to resolve into individual compounds. In this case, a change in polarity of solvent system can be explored to retard the running of the sample.

Applications of Thin layer Chromatography

1. Composition analysis of biomolecules/synthetic preparation

2. Quality testing of compound.

3. Identification of impurities in a sample

4. Progress of chemical reaction

5. Estimation of biomolecules

6. Bio-assay