Isolation of plasmid from bacteria: The different steps in isolation in bacterial plasmid is given in Figure 17.5.

STEP 1: The bacteria containing plasmid was grown in suitable culture media in high density (~0.8 optical density). Each Bacterial cell contains chromosomal DNA, plasmid DNA and cellular proteins. The bacterial culture is collected by centrifugation at the bottom and resuspended in the solution I containing 50 mM glucose, 25 mM TrisHCl pH 8.0, 10 mM EDTA pH 8.0.

Figure 17.5: Steps in Plasmid Purification from bacterial culture.