Quiz

Q1: Which enzyme can be used to make restriction enzyme generated sticky ends to blunt ends?

Q2: The enzyme used to remove 5’phosphate group?

Q3: Why type-II restriction enzymes are preferred for cloning?

Q4: Why ligation reaction is prefeered to incubate at low temperature (16⁰C) ?

Q5: Why BSA is added in restriction digestion reaction with EcoRI ?