Figure 32.2: Principle of Gel Filtration Chromatography.

Suppose the total column volume of a gel is V t and then it is given by-

V_g is the volume of gel matrix, V_i is the pore volume and V_o is the void volume. The volume of mobile phase flow to elute a column from a column is known as elution volume (V_e). The elution volum is related to the void volume and the distribution coefficient Kd as given below