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Figure 6.1 Diagrammatic illustration of targeted gene correction strategy:

In the figure 6.1 expressed ZFNs pairs bind to and break the target genomic locus close to the disease-causing mutation. ZFNs comprise of chimeric zinc fingers (yellow) and Fok1 endonuclease domains (blue). Further the repair template donor is used for the insertion of a DSB that stimulates homologous recombination.