Questions:

Q1. ------------- is the preferred way for viral isolation at most of the places.

Q2.Three types of cell culture are ------, ------------ and------.

Q3. Name any three susceptible cell lines.

Q4. viral particles sediment under a defined gravitational force called as -----------------.

Q5. ------------- and ------------------- are the types of sedimentation medium.

Q6. Virus infection to the cell can be visualized by the ----------- produced by the virus.

Q7. ----------------------- cell lines are derived from tumour cells.

Q8. The rate at which viral particles sediment under a defined gravitational force is called as------------------ and having a unit called the ---------------which is 10^-13 sec.

 

Answers:

• 1.  Cell culture

2.  Primary, Diploid and Continuous

3.  MDCK, Vero and LMH cells.

4.  Sedimentation Coefficient

5.  Sucrose cushions or gradient and CsCl 2 gradient centrifugation

6.  Cytopathic effects (CPE)

7.  Continuous/ Vero, Hep2 and Hela

8.  Sedimentation coefficient, Svedberg