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Susceptible cell lines:

Influenza virus- MDCK cells, Vero cells.

Paramyxoviruses- DF-1 cells, Vero cells.

Adenoviruses- HEK cells, HuH7 cells.

Herpesviruses- LMH cells.

Respiratory syncytia virus- Hep2 cells, Vero cells.

 

Figure 6.1. Virus induced CPE in cell culture

 

6.2. Purification of virus and components:

6.2.1. Ultracentrifugation:

The viruses are usually purified with the help of ultracentrifugation. The machine is capable of rotating the samples at 20,000-100,000 rpm under the density gradient of CsCl2 or sucrose. Density at which viruses neither sink nor float when suspended in a density gradient is called as buoyant density . The rate at which viral particles sediment under a defined gravitational force is called as sedimentation coefficient . The basic unit is the Svedberg (S) which is 10 -13 sec. The S value of a virus is used to estimate its molecular weight.