Fig. Selection of desired protein (Biopanning) : Bimolecule /receptor against which a protein/peptide is being designed was immobilized in the wells of a microtitre plate and probed with the phage peptide library. Unbound phages were removed by washing and then the bound phage were eluted with acid and amplified in liquid culture. The process was repeated 3-4 times and finally the phage was sequenced to identify the expressed protein sequence with high affinity. The process by which phage displaying desired protein sequence is enriched is called biopanning

Figure 4. Selection of desired protein (Biopanning). Bimolecule /receptor against which a protein/peptide is being designed was immobilized in the wells of a microtitre plate and probed with the phage peptide library. Unbound phages were removed by washing and then the bound phage were eluted with acid and amplified in liquid culture. The process was repeated 3-4 times and finally the phage was sequenced to identify the expressed protein sequence with high affinity. The process by which phage displaying desired protein sequence is enriched is called biopanning