Study questions
1. Define somaclonal variation. Discuss their achievements, advantages and limitations.
2. Which of the following would be likely or unlikely to result in somaclonal variation in micropropagated plants: (write in Yes/ No)
- (i) Propagation by axillary branching in the absence of plant growth regulators.
(ii) Somatic embryogenesis from a cell suspension culture.
(iii) Callus induction using 2,4-D (20 μM) followed by shoot organogenesis.
(iv) Regeneration from meristem explants.
3. What are the basic requirements of a somaclonal variant to be of commercial use?