Drawbacks

•  Presence of an assayable mutation within the host cell that can be compensated by the foreign gene expression which in most cases is not available. In addition, foreign genes may not fully compensate the mutations.

Applications

• This method can be used for the isolation of higher-eukaryotic genes (e.g. Drosophila topoisomerase II gene, a number of human RNA polymerase II transcription factors) from an organism.

•  It can also be possible in transgenic animals and plants to clone a specific gene from its functional homologue.

 

 

Bibliography

Benton WD, Davis RW. 1977. Screening λgt recombinant clones by hybridization to single plaques in situ. Science, 196: 180–182.

Benton WD, Davis RW. 1977. Screening λgt recombinant clones by hybridization to single plaques in situ. Science, 196 (4286): 180-182.

Campbell TN, Choy FYM. 2002. Approaches to Library Screening. J. Mol. Microbiol. Biotechnol. 4(6): 551-554.

Grunstein M, Hogness DS. 1975. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA , 72: 3961–3965.

Lodge J. 2007. Gene cloning: principles and applications. Taylor & Francis Group.

Primrose SB, Twyman RM. 2006. Principles of gene manipulation and genomics. 7 th ed. Blackwell Publishing.

Primrose SB, Tyman RM, Old RW. 2001. Principle of Gene Manipulation. 6 th ed. Wiley-Blackwell.

Probst FJ, Fridell RA, Raphael Yet al. 1998. Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene. Science, 280: 1444–1447.

Reece RJ. 2003. Analysis of Genes and Genomes. John Wiley & Sons, U.K.