Module 3 : NUCLEIC ACID HYBRIDIZATION AND AMPLIFICATION

Lecture 1 : Methods of Nucleic Acid Detection

Bibliography:

 

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Daxhelet, G.A., Coene, M.M., Hoet, P.P., and Cocito, C.G. 1989. Spectrofluorometry of dyes with DNAs of different base composition and conformation. Anal. Biochem. 179:401-403.

Fluorometric Quantitation of DNA Using Hoechst 33258 Cold Spring HarbProtoc; 2006; doi:10.1101/pdb.prot4458.

Gall, J. G. and Pardue, M. L. (1969). Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. Natl. Acad. Sci. USA 63, 378-383.

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Langer, P. R., Waldrop, A. A. and Ward, D. C. (1981). Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc. Natl. Acad. Sci. USA 78, 6633-6637.

Manuelidis, L., Langer-Safer, P. R. and Ward, D. C. (1982). High-resolution mapping of satellite DNA using biotin-labeled DNA probes. J. Cell Biol. 95, 619-625.

Molecular Cloning- A laboratory manual-David W. Russell & Joseph Sambrook.

Neidle, S. DNA minor-groove recognition by small molecules. 2001. Nat. Prod. Rep. 18:291-309.

Portugal, J. and Waring, M.J. (1988). Assignment of DNA binding sites for 4,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258): A comparative footprinting study. Biochem. Biophys. Acta 949:158-168.

Singer, R. H. and Ward, D. C. (1982). Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog. Proc. Natl. Acad. Sci. USA 79, 7331-7335.