Application:

• It is used to remove RNA contamination from DNA sample.

2-4.3.2 RibonucleaseH:

• Non-specific endoribonuclease that degrades RNA by hydrolytic mechanism from DNA/RNA duplex resulting in single stranded DNA.
  
  
• Enzyme bound divalent metal ion is a cofactor here. The product formed is 5' phosphorylated ssDNA.
  
  
• During cDNA library preparation from RNA sample, RNaseH enzyme is used to cleave RNA strand of DNA-RNA duplex.

Fig 2-4.3.2 : Schematic representation of cDNA preparation from mRNA

 

Bibliography:

• Boyer P. D. 1981. The Enzymes . Academic press Inc Ltd.
• Gerstein A.S. 2004. Molecular Biology Problem Solver: A Laboratory Guide. John Wiley & Sons.
• Gilbert S. F. 2010. Developmental Biology (9^th edition); Sinauer Associates, Inc.