Self Assessment Quiz


Q12.1. MM parameter for enzyme urease are Vm = 1.33 mol/s.L at E0 = 5g/L and Km = 0.266 mol/L.

Q12.1.1. Estimate time needed to remove 95% urea in a 0.5 lit batch vessel containing 0.001 g/L urease.


Q12.2. Competitive Inhibition kinetics : For the model below obtain rate Law
                            
                             E + S ⇔ ES  ; E + I ⇔ EI ; ES ⇒ P + E


Q12.3. Uncompetitive Inhibition kinetics
                           
                           
E + S ⇔ ES ; ES + I ⇔ IES ; ES ⇒ P + E


Q12.4. Non- Competitive inhibition : Obtain rate law
                           
                           
E + S ⇔ ES ;  ES + I ⇔ IES ;  ES ⇒ P + E ;   E + I ⇔ IE ;  IE + S ⇔ IES


Q12.5. Substrate Inhibition: Obtain rate law

                            E + S ⇔ ES ; ES + S ⇔ SES  ; ES ⇒ P + E


Q12.6 Multiple Steady states in Bioreactors (sourced from Fogler)
Data below is obtained from a CSTR of volume 1000 lit and feed enters at 3.2 lit/ s.


Q12.6.1 Show that the data given is consistent with substrate inhibition model of enzyme kinetics.

s 0 4 8 12 16 20 24 28 32 36 40 44 48 52
rs 0 0.17 0.14 0.11 0.09 0.075 0.07 0.06 0.055 0.05 0.045 0.04 0.037 0.035

S mmol/L, rs mmol/lit.min. Taken from P7-14 Scott Fogler Element of Chemical Reaction Engineering Prentice Hall 1986.


Q12.6.2. If the Reaction is carried out in a CSTR of volume 1000 lit and feed enters at 3.2 lit/ s.
Find all the steady states. If possible specify which are stable. S0 = 50 mmol/L.


Q12.6.3. What is the highest conversion possible if operated as above.