Figure 1: Expermental set-up are similar as in case of cation and anion exchange chromatography

Figure 2: A typical chromatogram for an enzyme purification. First protein peak is showing enzyme activity while the second peak is not active. Thus, enzyme is purified/partially purified in the first peak. Unwanted proteins are separated in second peak. Purity of the enzyme in first peak may be checked (we shall discuss methods in coming classes). In case of partial purification, fractions covering first peak may be pooled for next step of purification.

Animation showing ion-exchange chromatography

Quiz:

•  Explain how ion exchange chromatography may be used for concentrating a protein?