DNA extraction by silica based technology:

Silica gel based technology for extraction of DNA is the most popular, rapid and easy technology which yields ready to use DNA. This method yields highly purified DNA which can be used for any downstream application. It doesn't required extraction and precipitation of DNA. This method is based on selective adsorption of DNA on silica bed. The cell lysate was centrifuged to precipitate the cell debris and the supernatant was passed through silica bed. The buffer used for lysis ensures that only DNA adsorb on the silica bed and other components comes in flowthrouth. There are several kits in market based on this technology (Fig. 2)

Figure 2: Comparison of different DNA preparation methods.