As we have studied during earlier lectures, two dimensional gel electrophoresis (2D PAGE) is the specific technique for analysis of the whole protein expression profile of a given cell type or organism, under a set of specific conditions. In 2D PAGE, proteins in a sample are separated according to both isoelectric point (pI) and molecular weight (Mw), by a combination of isoelectric focusing and electrophoresis, respectively. Thus, 2D PAGE gel is characteristic of an organism at a given developmental stage and environmental condition. An ideal proteome data includes 2D PAGE gel image of an organism. This data may be available as tissue specific and developmental stage specific. Moreover, known functions/putative functions of all proteins of an organism are also mentioned. Moreover, all possible protein-protein interaction networks are also provided in many proteome databases.

Few Internet resources available:

Database	URL	Organism. Organelle/phenotype
Aarhus 2DPAGE Database	http:/biobase.dk/cgi-bin/cells	Human( Skin biology, bladder cancer, fibroblast), mouse(kidney)
Aberdeen 2D PAGE	http://www.abdn.ac.uk/~mmb023/2dhome.htm	Haemophilus influenza
Cyano2Dbase	http://www.kazusa.or.jp/cyano/cyano2D	Synechosystic cyanobacteria
ES cell-2D PAGE	http://www.dey.ac.uk/~dbl0nh I/2DPAGE	Embryonal stem cells
Harefield HSC 2D PAGE	http://www.harefeild.nthames.nhs.uk/nhil/protein	Human ( heart, endothelial cells), rat(heart), dog ( heart)
Maize genome database	http://moulon.moulon.inra.fr	Maize
SSI-2DPAGE	http://www.ssi.dk/en/forskning/tbimmun/ tbjemme.htm	M.tuberculosis
Proteome database system	http://www.mpiib-berlin.mpg.de/2D-PAGE/