Quiz-1

Q. What is the basis of primary classification of chromatography?

Ans. Primary classification of chromatography is based on the phenomenon involving the process of either partition or adsorption.

Q. What is partition or distribution coefficient?

Ans. Partition or distribution coefficient is the molar ratio of concentrations of analyte in stationary and mobile phase.

Partition or distribution coefficient K_d = X_A / X _B

Where X_A = Molar concentration of X in phase A.

X_B = Molar concentration of X in phase B.

Q. What are three molecular forces which affects the partition or distribution coefficient?

Ans .(a) Dispersion forces (b) Polar forces (c) Ionic forces

Q. What are the different types of column chromatography?

Ans. Different types of column chromatographies are as fallows

Adsorption chromatography, Partition chromatography, Ion-Exchange chromatography, Molecular Exclusion (Gel Filtration) chromatography, Affinity chromatography.

Q. What is capacity factor and what is the unit of capacity factor?

Ans. The additional time taken by an analyte to elute from the column relative to an excluded analyte that does not interact with the stationary phase is known as capacity factor (k'). It has no unit.

Capacity factor (k') = t_R− t _M / t _M = t _R '/ t _M