2-Dimential gel electrophoresis is generally undertaken when the global protein expression of an organism or a tissue is being investigated. Comparison of normal and experimental 2D gel data helps is identification of up-regulated or down regulated proteins (Fig. 2). Once protein band with altered expression is found, the band may be further analyzed for identification of protein by amino acid sequencing or peptide mass fingerprinting [we shall study these methods in coming lectures].

Figure 2: Comparison of normal and experimental 2D gel data for identification of up-regulated or down regulated proteins